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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP2E1 Antibody (C‑Terminus) IHC‑plus™ LS‑B1824
Note: This antibody replaces LS-C47057
CYP2E1 antibody LS-B1824 is an unconjugated rabbit polyclonal antibody to human CYP2E1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1824-50
50 µg (0.5 mg/ml)
Unavailable

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Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP2E1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
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Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CYP2E1 antibody LS-B1824 is an unconjugated rabbit polyclonal antibody to human CYP2E1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CYP2E1
CYP2E1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. P05181 NM_000773 NP_000764.1

CYP2E1 Antibody, 4-nitrophenol 2-hydroxylase Antibody, CPE1 Antibody, Cytochrome p450 IIe1 Antibody, Cytochrome P450-J Antibody, p450C2E Antibody, p450-J Antibody, CYP2E Antibody, CYPIIE1 Antibody, Cytochrome P450 2E1 Antibody


Specifications

Target
Human CYP2E1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYP2E1 antibody was raised against synthetic peptide from human CYP2E1.
Epitope
C-Terminus
Specificity
synthetic peptide corresponding to C-terminal residues of human CYP2E1 (Cytochrome P450 2E1)
Usage
Immunohistochemistry: LS-B1824 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1824 was determined to be 20 ug/ml.
Presentation
PBS, 0.01% sodium azide, 50% glycerol.
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.
Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.

Immunohistochemistry

Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.
Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.

Immunohistochemistry

Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.
Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.

Immunohistochemistry

Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.
Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1824 concentration 20 ug/ml.


Requested From: 
Date Requested: 6/22/2018

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