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Antibodies > CYP2E1 > LS-C313045
 
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Catalog Number Size Price
LS-C313045-10 10 µg $318 
LS-C313045-100 100 µg (0.5 mg/ml) $470 
CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP2E1 Antibody - CYP2E1 antibody. IHC(P): Rat Liver Tissue.
CYP2E1 Antibody - Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.
CYP2E1 Antibody - WB of CYP2E1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Mouse Liver Tissue Lysate.
CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP2E1 Antibody - CYP2E1 antibody. IHC(P): Rat Liver Tissue.
CYP2E1 Antibody - Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.
CYP2E1 Antibody - WB of CYP2E1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Mouse Liver Tissue Lysate.
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Polyclonal Rabbit anti‑Mouse CYP2E1 Antibody (aa94‑112, IHC, WB) LS‑C313045

Polyclonal Rabbit anti‑Mouse CYP2E1 Antibody (aa94‑112, IHC, WB) LS‑C313045

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Note: This antibody replaces LS-C388322
Antibody:
CYP2E1 Rabbit anti-Mouse Polyclonal (aa94-112) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313045-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYP2E1 Rabbit anti-Mouse Polyclonal (aa94-112) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYP2E1 antibody LS-C313045 is an unconjugated rabbit polyclonal antibody to CYP2E1 (aa94-112) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.
Target
Mouse CYP2E1
Synonyms
CYP2E1 | 4-nitrophenol 2-hydroxylase | CPE1 | Cytochrome p450 IIe1 | Cytochrome P450-J | p450C2E | p450-J | CYP2E | CYPIIE1 | Cytochrome P450 2E1
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of mouse CYP2E1 (94-112 aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.
Epitope
aa94-112
Specificity
Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP2E1
P05181 NM_000773 NP_000764.1

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IHC‑plus™ CYP2E1 Antibody LS‑B7286
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CYP2E1 ELISA Kit
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Reactivity: Mouse
Range: 3.12-200 ng/ml
See all CYP2E1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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