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Catalog Number Size Price
LS-C407761-10 10 µg $318 
LS-C407761-100 100 µg (0.5 mg/ml) $470 
CYP27B1 Antibody - CYP27B1 antibody IHC-paraffin. IHC(P): Human Kidney Cancer Tissue.
CYP27B1 Antibody - CYP27B1 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
CYP27B1 Antibody - CYP27B1 antibody Western blot. All lanes: Anti CYP27B1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: 293T Whole Cell Lysate at 40 ug. Predicted band size: 57 kD. Observed band size: 57 kD.
CYP27B1 Antibody - CYP27B1 antibody IHC-paraffin. IHC(P): Human Kidney Cancer Tissue.
CYP27B1 Antibody - CYP27B1 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
CYP27B1 Antibody - CYP27B1 antibody Western blot. All lanes: Anti CYP27B1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: 293T Whole Cell Lysate at 40 ug. Predicted band size: 57 kD. Observed band size: 57 kD.
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Polyclonal Rabbit anti‑Human CYP27B1 Antibody (aa475‑508, IHC, WB) LS‑C407761

Polyclonal Rabbit anti‑Human CYP27B1 Antibody (aa475‑508, IHC, WB) LS‑C407761

Antibody:
CYP27B1 Rabbit anti-Human Polyclonal (aa475-508) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407761-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CYP27B1 Rabbit anti-Human Polyclonal (aa475-508) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYP27B1 antibody LS-C407761 is an unconjugated rabbit polyclonal antibody to CYP27B1 (aa475-508) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Cited in 1 publication.
Target
Human CYP27B1
Synonyms
CYP27B1 | 1alpha(OH)ase | 25-OHD-1 alpha-hydroxylase | Calcidiol 1-monooxygenase | CYP1alpha | CYP27B | Cytochrome P450C1 alpha | Cytochrome p450 27B1 | Cytochrome P450VD1-alpha | p450c1 | VD3 1A hydroxylase | PDDR | VDD1 | CP2B | CYP1 | VDDR | VDDRI
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CYP27B1 (475-508 aa HFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR), different from the related mouse and rat sequences by six amino acids.
Epitope
aa475-508
Specificity
Kidney.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP27B1
O15528 NM_000785 NP_000776.1

LSBio Ratings

CYP27B1 Antibody (aa475-508) for IHC, WB/Western LS-C407761 has an LSBio Rating of
Publications (4)

Publications (1)

Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease. Megan L Gliozzi, Youssef Rbaibi, Kimberly R Long, Dario A Vitturi, Ora A Weisz. American journal of physiology. Cell physiology. 2019 November;317:C993-C1000.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024