(tested or 100% immunogen sequence identity)
- Western blot
- Functional Assay
CYP1A2 antibody was raised against recombinant human P450 1A2 expressed in E. coli.
Reactive with 0.2 ug of recombinant human P450 1A2. Only slightly cross-reacts with recombinant human P450 1A1after prolonged incubation. Does not cross-react with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Suitable for use in inhibition assays and Western Blot. A dilution of 1:2000 for Western blots will yield a positive result with 0.2 ug of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody according to the manufacturer's instructions. Inhibits approximately 80% of the ethoxyresorufin O-deethylase activity of human liver microsomes at a concentration of 10 mg IgG/nmol P450. Western Blot: Recognizes a band (P450 1A2) in human liver microsomes.
20 mM phosphate buffer, pH 8.0.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.