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Anti-CYP1A2 Antibody LS-C22611

Ordering

Wt. Vol. Conc. Price
- 500 µl - $495
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CYP1A2 Antibodies

Anti-CYP1A2 Antibody LS-C14776
Rabbit Polyclonal (IgG) to Human CYP1A2
Human, Rat
IHC - Frozen, Western blot
Unconjugated
Anti-CYP1A2 Antibody (clone 2C6) LS-C172346
Mouse Monoclonal [clone 2C6] (IgG1) to Human CYP1A2
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human CYP1A2
Human
Western blot, Functional Assay
Unconjugated

Details

Human CYP1A2
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • Western blot
  • Functional Assay

Specificity and Use

CYP1A2 antibody was raised against recombinant human P450 1A2 expressed in E. coli.
Reactive with 0.2 ug of recombinant human P450 1A2. Only slightly cross-reacts with recombinant human P450 1A1after prolonged incubation. Does not cross-react with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Suitable for use in inhibition assays and Western Blot. A dilution of 1:2000 for Western blots will yield a positive result with 0.2 ug of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody according to the manufacturer's instructions. Inhibits approximately 80% of the ethoxyresorufin O-deethylase activity of human liver microsomes at a concentration of 10 mg IgG/nmol P450. Western Blot: Recognizes a band (P450 1A2) in human liver microsomes.

Packaging

20 mM phosphate buffer, pH 8.0.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CYP1A2

P05177 NM_000761 NP_000752.2

CYP1A2 Antibody, Aryl hydrocarbon hydroxylase Antibody, Cytochrome P(3)450 Antibody, Cytochrome P450 1A2 Antibody, Cytochrome P450 4 Antibody, p450 form 4 Antibody, p3-450 Antibody, p450(PA) Antibody, CP12 Antibody, CYPIA2 Antibody, Cytochrome p450 ia2 Antibody, Cytochrome P450-P3 Antibody, Dioxin-inducible P3-450 Antibody

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen.

Requested From: United States
Date Requested: 12/11/2016

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