Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Rat (tested or 100% immunogen sequence identity)
IHC - Frozen
Western blot (1:1000 - 1:4000)
Specificity and Use
CYP1A2 antibody was raised against synthetic peptide from human CYP1A2.
Reacts with human cytochrome P450 enzyme CYP1A2 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes. By Western blot recognizes a protein with MW ~54kD. Species cross-reactivity: Human and rat.
Western blot: 1:1000-1:4000. Immunohistochemistry on frozen and formaldehyde treated sections.
No preservative added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen.