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Anti-CYP19 / Aromatase Antibody (aa475-486) IHC-plus™ LS-B3021

Note: This antibody replaces LS-C54539
Catalog Size Price
LS-B3021-50 50 µg (0.5 mg/ml) Unavailable

Most Popular CYP19 / Aromatase Antibodies

Anti-CYP19 / Aromatase Antibody (aa400-502) IHC-plus™ LS-B521
Rabbit Polyclonal to Human CYP19 / Aromatase
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CYP19 / Aromatase Antibody IHC-plus™ LS-B2816
Rabbit Polyclonal to Human CYP19 / Aromatase
Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CYP19 / Aromatase Antibody (aa475-486) IHC-plus™ LS-B3021
Goat Polyclonal to Human CYP19 / Aromatase
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CYP19 / Aromatase Antibody (aa221-270) LS-C119485
Rabbit Polyclonal (IgG) to Human CYP19 / Aromatase
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CYP19 / Aromatase Antibody (aa376-390, clone H4) IHC-plus™ LS-B7706
Mouse Monoclonal [clone H4] (IgG2a) to Human CYP19 / Aromatase
Human, Rat, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Goat Polyclonal to Human CYP19 / Aromatase
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CYP19 / Aromatase
Goat
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (4 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:128000)

Specificity and Use

Synthetic peptide C-HDLSLHPDETKN from an internal region of human CYP19A1 / Aromatase (NP_000094.2; NP_112503.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
LS-E26615 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3021. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa475-486
Human CYP19A1 / Aromatase. This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical
Immunohistochemistry: LS-B3021 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3021 was determined to be 4 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CYP19 / Aromatase

P11511 NM_031226 NP_000094.2

CYP19A1 Antibody, ARO1 Antibody, CYAR Antibody, CPV1 Antibody, CYPXIX Antibody, Cytochrome P-450AROM Antibody, Cytochrome P450 19A1 Antibody, CYP19 Antibody, Estrogen synthase Antibody, Estrogen synthetase Antibody, P-450AROM Antibody, ARO Antibody, Aromatase Antibody

CYP19 / Aromatase is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis.

Immunohistochemistry

Immunohistochemistry
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3021 concentration 4 ug/ml.

Western blot

Western blot
Antibody (0.1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 8/17/2017

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