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CYFIP1 Antibody (aa1159‑1208) LS‑C151744 Best Seller
CYFIP1 antibody LS-C151744 is an unconjugated rabbit polyclonal antibody to CYFIP1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C151744-50
50 µg (1 mg/ml)
Unavailable

Popular CYFIP1 Products

Detection of Human CYFIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CYFIP1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CYFIP1 was also immunoprecipitated by rabbit anti-CYFIP1 antibody BL10812, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Species: Human
Applications: Western blot, Immunoprecipitation
All lanes : Anti-cyfip1 Antibody at 1:2000 dilution Lane 1: ZF4 whole cell lysates Lane 2: Zebrafish lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 145 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Zebrafish
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of CYFIP1 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: PC12 whole cell lysate.
Species: Human, Mouse, Rat
Applications: Western blot
Immunofluorescence staining of A549 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA

Product Description

CYFIP1 antibody LS-C151744 is an unconjugated rabbit polyclonal antibody to CYFIP1 from human, mouse, rat and other species. Validated for WB.
About CYFIP1
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA By similarity. Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Q7L576 D38549 BAA07552.1

CYFIP1 Antibody, KIAA0068 Antibody, p140SRA-1 Antibody, Selective hybridizing clone Antibody, Sra-1 Antibody, SHYC Antibody


Specifications

Target
Human CYFIP1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Goat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CYFIP1 antibody was raised against synthetic peptide located between aa1159-1208 of human CYFIP1 (Q7L576, NP_055423). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Salmon, Medaka, Pufferfish, Zebrafish (100%); Goat, Elephant, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Stickleback (92%).
Blocking Peptide
LS-E23526 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C151744. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1159-1208
Specificity
Human CYFIP1
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CYFIP1 antibody Western Blot of COLO205.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CYFIP1 antibody Western Blot of COLO205. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CYFIP1 antibody Western Blot of COLO205.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CYFIP1 antibody Western Blot of COLO205. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CYFIP1 antibody Western Blot of COLO205.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CYFIP1 antibody Western Blot of COLO205. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CYFIP1 antibody Western Blot of COLO205.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CYFIP1 antibody Western Blot of COLO205. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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