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Anti-CYFIP1 Antibody (aa1159-1208) LS-C151744

Catalog Size Price
LS-C151744-50 50 µg (1 mg/ml) Unavailable

Related Products

13 CYFIP1 Antibodies

Most Popular CYFIP1 Antibodies

Anti-CYFIP1 Antibody (aa1203-1253) LS-C289702
Rabbit Polyclonal (IgG) to Human CYFIP1
Human
Western blot, Immunoprecipitation
Unconjugated
Detection of Human CYFIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CYFIP1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CYFIP1 was also immunoprecipitated by rabbit anti-CYFIP1 antibody BL10812, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Anti-CYFIP1 Antibody (aa524-558) LS-C357401
Rabbit Polyclonal to Zebrafish CYFIP1
Zebrafish
Western blot, ELISA
Unconjugated
All lanes : Anti-cyfip1 Antibody at 1:2000 dilution Lane 1: ZF4 whole cell lysates Lane 2: Zebrafish lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 145 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CYFIP1 Antibody LS-C680345
Rabbit Polyclonal (IgG) to Human CYFIP1
Human
Immunofluorescence, ELISA
Unconjugated
Immunofluorescence staining of A549 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Anti-CYFIP1 Antibody LS-C680346
Rabbit Polyclonal (IgG) to Human CYFIP1
Human
ELISA
Unconjugated
Anti-CYFIP1 Antibody LS-C680348
Rabbit Polyclonal (IgG) to Human CYFIP1
Human
ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human CYFIP1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human CYFIP1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Goat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

CYFIP1 antibody was raised against synthetic peptide located between aa1159-1208 of human CYFIP1 (Q7L576, NP_055423). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Salmon, Medaka, Pufferfish, Zebrafish (100%); Goat, Elephant, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Stickleback (92%).
LS-E23526 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C151744. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa1159-1208
Human CYFIP1

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CYFIP1

Q7L576 D38549 BAA07552.1

CYFIP1 Antibody, KIAA0068 Antibody, p140SRA-1 Antibody, Selective hybridizing clone Antibody, Sra-1 Antibody, SHYC Antibody

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA By similarity. Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA.

Western blot

CYFIP1 antibody Western Blot of COLO205.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CYFIP1 antibody Western Blot of COLO205. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/25/2018

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