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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYCS / Cytochrome c Antibody (clone 7H8.2C12) IHC‑plus™ LS‑B3673
Note: This antibody replaces LS-C63127
Cytochrome c antibody LS-B3673 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3673-50
50 µg (1 mg/ml)
Unavailable

Popular CYCS / Cytochrome c Products

Antibody
Species: Human, Mouse, Rat, Dog
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Western blot of Cytochrome c antibody LS-C48137.
Species: Pig, Human, Mouse, Rat
Applications: Western blot
Anti-Cytochrome c antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2141 concentration 5 ug/ml.
Species: Pigeon, Human, Mouse, Rat, Hamster, Horse, Drosophila
Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Horse, Human, Bovine, Dog
Applications: Immunoprecipitation

Product Description

Cytochrome c antibody LS-B3673 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CYCS / Cytochrome c
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. P99999 NM_018947 NP_061820.1

CYCS Antibody, CYC Antibody, HCS Antibody, THC4 Antibody, Cytochrome c Antibody, Cytochrome c, somatic Antibody


Specifications

Target
Horse CYCS / Cytochrome c
Host
Mouse
Reactivity
Horse, Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [7H8.2C12]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Horse cytochrome c.
Specificity
Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.
Usage
Immunohistochemistry: LS-B3673 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3673 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.2, 50% glycerol, 0.09% azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/24/2018

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