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Anti-CYCS / Cytochrome c Antibody (clone 7H8.2C12) IHC-plus™ LS-B3673

Note: This antibody replaces LS-C63127

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CYCS / Cytochrome c Antibodies

Anti-CYCS / Cytochrome c Antibody LS-C48137
Mouse Monoclonal (IgG2b) to Pig CYCS / Cytochrome c
Pig, Human, Mouse, Rat
Western blot
Unconjugated
Western blot Image
Anti-CYCS / Cytochrome c Antibody (clone 2B5.F8) LS-C63083
Mouse Monoclonal [clone 2B5.F8] (IgG2a) to Horse CYCS / Cytochrome c
Horse, Human, Bovine, Dog
Immunoprecipitation
Unconjugated
Anti-CYCS / Cytochrome c Antibody (Internal, clone EPR1327) IHC-plus™ LS-B6577
Rabbit Monoclonal [clone EPR1327] to Human CYCS / Cytochrome c
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7H8.2C12] (IgG2b) to Horse CYCS / Cytochrome c
Horse, Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated

Details

Horse CYCS / Cytochrome c
Mouse
Horse, Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [7H8.2C12]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

CYCS / Cytochrome c antibody was raised against horse cytochrome c.
Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.
Immunohistochemistry: LS-B3673 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3673 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, 50% glycerol, 0.09% azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CYCS / Cytochrome c

P99999 NM_018947 NP_061820.1

CYCS Antibody, CYC Antibody, HCS Antibody, THC4 Antibody, Cytochrome c Antibody, Cytochrome c, somatic Antibody

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Cytochrome c antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Cytochrome c antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3673 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/7/2016

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