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Catalog Number Size Price
LS-B9227-50 50 µg (0.5 mg/ml) $485 
CYB5R3 / B5R Antibody - Anti-CYB5R3 / B5R antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
CYB5R3 / B5R Antibody - CYB5R3 antibody (0.01 ug/ml) staining of Human Umbilical Cord lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CYB5R3 / B5R Antibody - Anti-CYB5R3 / B5R antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
CYB5R3 / B5R Antibody - CYB5R3 antibody (0.01 ug/ml) staining of Human Umbilical Cord lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human CYB5R3 / B5R Antibody (aa317‑330, IHC, WB) LS‑B9227

IHC‑plus™ Polyclonal Goat anti‑Human CYB5R3 / B5R Antibody (aa317‑330, IHC, WB) LS‑B9227

Note: This antibody replaces LS-C139733
Antibody:
CYB5R3 / B5R Goat anti-Human Polyclonal (aa317-330) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B9227-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CYB5R3 / B5R Goat anti-Human Polyclonal (aa317-330) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B5R antibody LS-B9227 is an unconjugated goat polyclonal antibody to human B5R (CYB5R3) (aa317-330). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CYB5R3 / B5R
Synonyms
CYB5R3 | B5R | Cytochrome b5 reductase 3 | DIA1 | Diaphorase-1 | Cytochrome b5 reductase | NADH-cytochrome b5 reductase | NADH-cytochrome b5 reductase 3
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence CLPNLDHVGHPTER, from the C-Terminus of protein sequence according to NP_000389.1NP_001123291.1NP_001165131.1.
Epitope
aa317-330
Specificity
Human CYB5R3. This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 37kD band observed in Human Placenta, Testis and Umbilical Cord lysates (calculated MW of 38.2kD according to NP_001165131.1). Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYB5R3 / B5R
P00387 NM_007326 NP_015565.1

Validation

CYB5R3 / B5R Antibody - Anti-CYB5R3 / B5R antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYB5R3 / B5R antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYB5R3 / B5R antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ CYB5R3 / B5R Antibody (aa317-330) for IHC, WB/Western LS-B9227 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024