(tested or 100% immunogen sequence identity)
Protein G purified
CXCR5 antibody was raised against recombinant corresponding to mouse CXCR5 expressed in E. coli (Q04683)
Flow Cytometry: 2.5ug per 10e6 cells; Mouse splenocytes werestained with catalog #LS-C143773 followed by PerCP-conjugatedanti-Rat IgG F(ab')2 secondary antibody and a mouseB220/CD45R PE-conjugated monoclonal antibody.Immunocytochemistry: 8-25ug/ml; CXCR5 was detected inimmersion fixed A20 mouse B cell lymphoma cell line usingcatalog #LS-C143773 at 10ug/ml for 3 hours at roomtemperature. Cells were stained using the fluorescent-conjugatedanti-Rat IgG secondary antibody and counterstained with DAPI.Staining was localized to cell surfaces and cytoplasm.Optimal dilutions to be determined by the researcher.
Lyophilized from PBS, pH 7.4, 5% trehalose
200 µl Sterile PBS
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.