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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CXCR5 Antibody (clone 614641) LS‑C143773
Antibody
CXCR5 antibody LS-C143773 is an unconjugated rat monoclonal antibody to mouse CXCR5. Validated for ELISA, Flow and ICC.
Catalog
Size
Price
LS-C143773-100
100 µg (0.5 mg/ml)
Unavailable

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Product Description

CXCR5 antibody LS-C143773 is an unconjugated rat monoclonal antibody to mouse CXCR5. Validated for ELISA, Flow and ICC.
About CXCR5
CXCR5, a Chemokine Receptor, also called Burkitt's lymphoma receptor 1, is required for normal development of Peyer's patches, inguinal lymph nodes, and splenic follicles. CXCR5 binds B-lymphocyte chemoattractant (BLC) and is involved in B-cell homing to follicles in lymph nodes and spleen. Two isoforms are produced by alternative splicing. P32302 NM_001716 NP_001707.1

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Specifications

Target
Mouse CXCR5
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [614641]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Flow Cytometry
  • ELISA
Immunogen
CXCR5 antibody was raised against recombinant corresponding to mouse CXCR5 expressed in E. coli (Q04683)
Specificity
Mouse CXCR5.
Usage
Flow Cytometry: 2.5ug per 10e6 cells; Mouse splenocytes werestained with catalog #LS-C143773 followed by PerCP-conjugatedanti-Rat IgG F(ab')2 secondary antibody and a mouseB220/CD45R PE-conjugated monoclonal antibody.Immunocytochemistry: 8-25ug/ml; CXCR5 was detected inimmersion fixed A20 mouse B cell lymphoma cell line usingcatalog #LS-C143773 at 10ug/ml for 3 hours at roomtemperature. Cells were stained using the fluorescent-conjugatedanti-Rat IgG secondary antibody and counterstained with DAPI.Staining was localized to cell surfaces and cytoplasm.Optimal dilutions to be determined by the researcher.
Presentation
Lyophilized from PBS, pH 7.4, 5% trehalose
Reconstitution
200 µl Sterile PBS
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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