Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils.
Western Blot analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PPBP is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.