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Anti-CXCL7 / PPBP Antibody (aa40-1) LS-C150178

Catalog	Size	Price
LS-C150178-25	25 µg	Unavailable
LS-C150178-100	100 µg	Unavailable

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Anti-CXCL7 / PPBP Antibody IHC-plus™ LS-B7394

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Anti-CXCL7 / PPBP Antibody IHC-plus™ LS-B13073

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Reactivity:	Human, Mouse
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Antibody:	Goat Polyclonal (IgG) to Mouse CXCL7 / PPBP
Reactivity:	Mouse
Application:	IHC, Western blot
Format:	Unconjugated

Details

Target:	Mouse CXCL7 / PPBP
Host:	Goat
Reactivity:	Mouse (tested or 100% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

IHC (5 - 15 µg/ml)
Western blot (0.1 µg/ml)

Specificity and Use

Immunogen:	CXCL7 / PPBP antibody was raised against e. coli-derived recombinant mouse CXCL7/Thymus Chemokine1 Lys40-Tyr1 13 Accession # AAG36786.
Epitope:	aa40-1
Specificity:	Detects CXCL7/Thymus Chemokine-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with.
Usage:	Western Blot, 0.1 µg/ml, Recombinant Mouse CXCL7/Thymus Chemokine-1. Immunohistochemistry, 5-15 µg/ml.

Packaging

Presentation:	Lyophilized from 0.2 um filtered solution in PBS, trehalose
Reconstitution:	Sterile PBS. Resuspend to 500 ul
Storage:	Store at -20 to -70 C. Stable for 12 months from date of receipt.
Restrictions:	For research use only.

About CXCL7 / PPBP

Uniprot: P02775 NCBI: NM_002704 NP_002695.1

Synonyms: PPBP Antibody, Beta-thromboglobulin Antibody, B-TG1 Antibody, Beta-TG Antibody, C-X-C motif chemokine 7 Antibody, CTAP3 Antibody, CXCL7 Antibody, CTAP-III Antibody, CXC chemokine ligand 7 Antibody, NAP-2 Antibody, NAP-2-L1 Antibody, PBP Antibody, LA-PF4 Antibody, LDGF Antibody, SCYB7 Antibody, Small-inducible cytokine B7 Antibody, Small inducible cytokine B7 Antibody, THBGB1 Antibody, Thrombocidin 2 Antibody, TC2 Antibody, TGB Antibody, Thrombocidin 1 Antibody, Thromboglobulin, beta-1 Antibody, CTAPIII Antibody, MDGF Antibody, Platelet basic protein Antibody, SCAR10 Antibody, TC1 Antibody, THBGB Antibody

Description: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils.

(More About CXCL7 / PPBP)

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Technical Help Review

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Immunohistochemistry

CXCL7/Thymus Chemokine-1 in Mouse Thymus. CXCL7/Thymus Chemokine-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody at 5 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes.

Neutralization

Chemotaxis Induced by CXCL7/Thymus Chemokine-1 and Neutralization by Mouse CXCL7/Thymus Chemokine-1 Antibody. Recombinant Mouse CXCL7/Thymus Chemokine-1 chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine-1 (40 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.8-4 ug/ml.

Requested From:
Date Requested: 8/21/2017

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