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Anti-CXCL1 / GRO Alpha Antibody LS-C104351

Note: This antibody replaces LS-C15621

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CXCL1 / GRO Alpha Antibodies

Anti-CXCL1 / GRO Alpha Antibody LS-C48831
Rabbit Polyclonal to Rat CXCL1 / GRO Alpha
Rat, Mouse
IHC, Western blot, Immunoprecipitation
Unconjugated
Western blot Image
Anti-CXCL1 / GRO Alpha Antibody (aa36-107, clone 7A11) LS-C125418
Mouse Monoclonal [clone 7A11] (IgG) to Human CXCL1 / GRO Alpha
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CXCL1 / GRO Alpha
Human
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Human CXCL1 / GRO Alpha
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (1.5 - 3 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CXCL1 / GRO Alpha antibody was raised against e.coli derived recombinant Human GRO/MGSA.
Human GRO/MGSA
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml-0.150 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human GRO/MGSA (100 ng/ml), a concentration of 1.5-3 ug/ml of this antibody is required. ELISA: To detect Human GRO/MGSA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO/MGSA (LS-C104350) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO/MGSA. Western Blot: To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CXCL1 / GRO Alpha

P09341 NM_001511 NP_001502.1

CXCL1 Antibody, C-X-C motif chemokine 1 Antibody, Fibroblast secretory protein Antibody, FSP Antibody, GRO1 Antibody, GROa Antibody, Growth-regulated alpha protein Antibody, GRO-alpha(1-73) Antibody, MGSA Antibody, MGSA-a Antibody, Small inducible cytokine b1 Antibody, GRO Antibody, MGSA alpha Antibody, NAP-3 Antibody, SCYB1 Antibody

GRO Alpha (CXCL1), a small cytokine belonging to the CXC chemokine family, secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. GRO alpha is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity.

Immunohistochemistry

Immunohistochemistry
IHC of GRO / CXCL1 antibody LS-C104351. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of GRO / CXCL1 antibody LS-C104351. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of GRO / CXCL1 antibody LS-C104351. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of GRO / CXCL1 antibody LS-C104351. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of GRO / CXCL1 antibody LS-C104351. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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