Human Cullin 4A / CUL4A

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated followed by sterile filtration

Unmodified

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cul4A coupled to KLH.

aa1-25

Reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:500 - 1:1000)
• Immunoprecipitation (1:500)
• ELISA (1:2000 - 1:10000)

Immunohistochemistry: LS-B360 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B360 was determined to be 1:500.

Antiserum, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13619 NCBI: NM_003589 NP_003580.1