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Anti-CUL1 / Cullin 1 Antibody (C-Terminus) IHC-plus™ LS-B2448

Note: This antibody replaces LS-C6247

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CUL1 / Cullin 1 Antibodies

Anti-CUL1 / Cullin 1 Antibody (clone AS97.1) LS-C63155
Mouse Monoclonal [clone AS97.1] (IgG2a) to Human CUL1 / Cullin 1
Human, Monkey, Mouse, Rat, Bovine, Rabbit
Western blot
Unconjugated
Anti-CUL1 / Cullin 1 Antibody (C-Terminus) IHC-plus™ LS-B2448
Mouse Monoclonal (IgG1,k) to Human CUL1 / Cullin 1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

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Mouse Monoclonal (IgG1,k) to Human CUL1 / Cullin 1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CUL1 / Cullin 1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

CUL1 / Cullin 1 antibody was raised against a synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.
C-Terminus
Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.
Immunohistochemistry: LS-B2448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2448 was determined to be 10 ug/ml. Immunoprecipitation: 5-10 ug/ IP reaction.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CUL1 / Cullin 1

Q13616 NM_003592 NP_003583.2

CUL1 Antibody, Cullin 1 Antibody, Cullin-1 Antibody, CUL-1 Antibody

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Immunohistochemistry

Immunohistochemistry
Anti-CUL1 / Cullin 1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2448 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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