Human CTNNB1 / Beta Catenin

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic phosphopeptide derived from a region of human -catenin that contains serine 45. The sequence is conserved in mouse, rat and frog.

pSer45

Human -catenin. Mouse, rat and frog -catenin (100% homologous) have not been tested, but are expected to react.

• Western blot (1:1000 - 1:2000)
• ELISA

The antibody has been used for Western blotting applications. b-catenin (92kD) is a multifunctional protein involved in cell-cell adhesion, intracellular. signaling and gene transcription. b-catenin plays a key role in the Wnt pathway which mediates numerous developmental processes. Deregulation of the Wnt pathway results in the accumulation of b-catenin in the nucleus. Stabilizing mutations of several serine/threonine residues (more frequently of serine 45) have been found in multiple tumors. Phosphorylation of b-catenin on serine 45 by casein kinase I (CKI) primes its subsequent phosphorylation of residues 33, 37 and 41 by glycogen synthase-3b (GSK-3b) and results in its degradation. Applications: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:2000.

1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35222 NCBI: NM_001904 NP_001895.1