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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CTNNB1 / Beta Catenin Antibody (clone 9F6) LS‑C172671
Beta Catenin antibody LS-C172671 is an unconjugated mouse monoclonal antibody to Beta Catenin (CTNNB1) from human and dog. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C172671-100
100 µl (0.37 mg/ml)
Unavailable

Popular CTNNB1 / Beta Catenin Products

CTNNB1 / Beta Catenin antibody LS-C31415 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-CTNNB1 / Beta Catenin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1592 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Western blot
Anti-CTNNB1 / Beta Catenin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4123 dilution 1:200.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot analysis of extracts from MCF-7 cells.
Species: Human
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Western blot of Phospho-Catenin-beta (S552) antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

Beta Catenin antibody LS-C172671 is an unconjugated mouse monoclonal antibody to Beta Catenin (CTNNB1) from human and dog. Validated for Flow, IF, IHC and WB.
About CTNNB1 / Beta Catenin
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. P35222 NM_001904 NP_001895.1

CTNNB1 Antibody, Beta-catenin Antibody, Catenin beta-1 Antibody, CTNNB Antibody, Beta Catenin Antibody


Specifications

Target
Human CTNNB1 / Beta Catenin
Host
Mouse
Reactivity
Human, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [9F6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.
Specificity
Human Beta Catenin
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunofluorescence

Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.
Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunofluorescence

Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.
Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunofluorescence

Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.
Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTNNB1 mouse monoclonal antibody.

Immunofluorescence

Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.
Anti-CTNNB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/21/2018

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