Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1,k) to Mouse CTNNA1 / Catenin Alpha-1
Mouse, Human, Rat, Chicken, Xenopus
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Mouse Monoclonal (IgG1,k) to Human CTNNA1 / Catenin Alpha-1
Human, Mouse, Rat, Dog
ICC, Western blot, Immunoprecipitation, ELISA
Human CTNNA1 / Catenin Alpha-1
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
ICC (0.1 - 1 µg/ml)
Western blot (0.2 µg/ml)
ELISA (0.05 µg/ml)
Specificity and Use
Recombinant human alpha-catenin.
Recognizes human alpha-catenin. Detects a band of ~102kD. Species cross-reactivity: mouse, rat, and canine.
Suitable for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blot. Western Blot: 0.2 ug/ml for HRP/ECL detection. Immunoprecipitation: 1-10 ug per 1E6 vanadate treated A431 cells. Immunocytochemistry: 0.1-1 ug/ml; alpha catenin may tolerate formaldehyde fixation. ELISA: 0.05 ug/ml. Positive control: C2069-44H1: MCF-7 Cell Lysate, Untreated.
PBS, 0.09% azide, G, sucrose, 40% glycerol.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions.