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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CTNNA1 / Catenin Alpha‑1 Antibody (C‑Terminus, clone 7A4) LS‑C12447
Catenin Alpha-1 antibody LS-C12447 is an unconjugated mouse monoclonal antibody to Catenin Alpha-1 (CTNNA1) from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C12447-100
100 µg
$605

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Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

Catenin Alpha-1 antibody LS-C12447 is an unconjugated mouse monoclonal antibody to Catenin Alpha-1 (CTNNA1) from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB.
About CTNNA1 / Catenin Alpha-1
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. P35221 NM_001903 NP_001894.2

CTNNA1 Antibody, AlphaE-catenin Antibody, Alpha-E-catenin Antibody, Alpha(E)-catenin Antibody, Alpha-1 catenin Antibody, Alpha-catenin Antibody, Cadherin-associated protein Antibody, Alpha E-catenin Antibody, CAP102 Antibody, Catenin alpha-1 Antibody


Specifications

Target
Mouse CTNNA1 / Catenin Alpha-1
Host
Mouse
Reactivity
Mouse, Human, Rat, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [7A4]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence (2 - 5 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the C-terminus of mouse a-catenin.
Epitope
C-Terminus
Specificity
Recognizes mouse a-catenin. Does not cross-react with the related beta- and gamma-catenin proteins. Species cross-reactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol, NIH 3T3 cells.
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 2-5 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1 ug/ml. Immunohistochemistry ( formalin- fixed, paraffin-embedded tissue): 2-10 ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic. Immunoprecipitation: 5 ug. Positive control: Pancreas/breast tumor/heart.Tested with Xenopus samples using Western blot only.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/20/2018

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