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Anti-CTNNA1 / Catenin Alpha-1 Antibody (C-Terminus, clone 7A4) LS-C12447

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Mouse Monoclonal [clone 7A4] (IgG1,k) to Mouse CTNNA1 / Catenin Alpha-1
Mouse, Human, Rat, Chicken, Xenopus
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Mouse CTNNA1 / Catenin Alpha-1
Mouse
Mouse, Human, Rat, Chicken, Xenopus (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [7A4]
Immunoaffinity purified

Applications

IHC - Paraffin, Immunofluorescence (2 - 5 µg/ml), Western blot (1 µg/ml), Immunoprecipitation, ELISA (0.1 - 1 µg/ml)

Specificity and Use

CTNNA1 / Catenin Alpha-1 antibody was raised against synthetic peptide derived from the C-terminus of mouse a-catenin.
C-Terminus
Recognizes mouse a-catenin. Does not cross-react with the related beta- and gamma-catenin proteins. Species cross-reactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol, NIH 3T3 cells.
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 2-5 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1 ug/ml. Immunohistochemistry ( formalin- fixed, paraffin-embedded tissue): 2-10 ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic. Immunoprecipitation: 5 ug. Positive control: Pancreas/breast tumor/heart.Tested with Xenopus samples using Western blot only.

Packaging

PBS, pH 7.2, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CTNNA1 / Catenin Alpha-1

P35221 NM_001903 NP_001894.2

CTNNA1 Antibody, AlphaE-catenin Antibody, Alpha-E-catenin Antibody, Alpha(E)-catenin Antibody, Alpha-1 catenin Antibody, Alpha-catenin Antibody, Cadherin-associated protein Antibody, Alpha E-catenin Antibody, CAP102 Antibody, Catenin alpha-1 Antibody

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions.

Requested From: United States
Date Requested: 9/26/2016

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