Human CTLA4 / CD152

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

BNI3

R. Phycoerythrin. Also available Unconjugated.

Affinity purified

Unmodified

Human CTLA-4/human IgG heavy chain fusion protein.

Recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86. Is able to block ligand binding on the Raji B-cell line and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Further studies indicate that clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98,. The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2. CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface. CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases.

• Flow Cytometry (1:1)
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 20 ul of the suggested working dilution to label 5x10^5 cells in 100 ul. Method sheets are available on request.

PBS, 0.1% sodium azide, 2% BSA.

Store at 4°C. Do not freeze. beforeStore undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16410 NCBI: NM_005214 NP_005205.2