Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
IHC - Paraffin
CTLA4 / CD152 antibody was raised against human CTLA-4/human IgG heavy chain fusion protein.
Recognizes human CD152 cell surface antigen, also known as CTLA-4 (Cytolytic T Lymphocyte Associated Antigen 4).
Suitable for use in Flow Cytometry, Immunohistochemistry with frozen tissues. Flow Cytometry: Use 21 ul of the suggested working dilution to label 1E6 cells in 100ul. Membrane permeabilization may be applicable for studies with this antibody. We recommend the use of a suitable Fixation/Permeabilization medium for this purpose. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 2 mg/ml BSA, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
CTLA4 / CD152 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.