LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CTCF Antibody (aa650‑700) LS‑C47284
Note: This antibody replaces LS-C78028
CTCF antibody LS-C47284 is an unconjugated rabbit polyclonal antibody to CTCF from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C47284-100
100 µg
Unavailable

Popular CTCF Products

Anti-CTCF antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1518 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-CTCF antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10616 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Detection of Human and Mouse by Immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- used at a dilution of 1:100 (2 Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CTCF mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Antibody staining CTCF in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CTCF antibody LS-C47284 is an unconjugated rabbit polyclonal antibody to CTCF from human, mouse, rat and other species. Validated for WB.
About CTCF
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. P49711 NM_006565 NP_006556.1

CTCF Antibody, 11-zinc finger protein Antibody, CCCTC-binding factor Antibody, Transcriptional repressor CTCF Antibody, CTCFL paralog Antibody


Specifications

Target
Human CTCF
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
Immunogen
CTCF antibody was raised against a portion of amino acids 650-700 of human CTCF.
Epitope
aa650-700
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, _b, _c, _d), rhesus monkey, dog, mouse and rat.
Presentation
PBS, 0.2% gelatin, 0.05% sodium azide.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.

Western blot

Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.

Western blot

Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.

Western blot

Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
Western blot of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.


Requested From: 
Date Requested: 6/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number