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Anti-CTCF Antibody (aa150-200) IHC-plus™ LS-B3184

Note: This antibody replaces LS-C78025
See our new CTCF ELISA Kits
Mouse CTCF ELISA Kit - LS-F11234
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg - - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CTCF Antibody LS-B3184 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CTCF antibody IHC of human thymus.
Immunohistochemistry
Anti-CTCF antibody IHC of human kidney.
Immunohistochemistry
Anti-CTCF antibody IHC of human breast.

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Rabbit Polyclonal to Human CTCF
Human
IHC - Paraffin, Immunoprecipitation
Unconjugated

Details

Human CTCF
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Immunoprecipitation

Specificity and Use

CTCF antibody was raised against a synthetic peptide mapping to a region between residues 150 and 200 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).
aa150-200
Species cross-reactivity: Human. Other species have not been tested. Reactivity with mouse (but not X. laevis) CTCF is expected owing to similarity of sequence between this ortholog and human CTCF at the site to which the epitope maps.
Immunohistochemistry: LS-B3184 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3184 was determined to be 5 mg/ml.

Packaging

Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CTCF

P49711 NM_006565 NP_006556.1

CTCF Antibody, 11-zinc finger protein Antibody, CCCTC-binding factor Antibody, Transcriptional repressor CTCF Antibody, CTCFL paralog Antibody


Immunohistochemistry

Immunohistochemistry
Anti-CTCF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CTCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CTCF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 7/1/2015

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