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Anti-CTCF Antibody (aa150-200) IHC-plus™ LS-B3184

Note: This antibody replaces LS-C78025


Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CTCF Antibodies

Anti-CTCF Antibody (N-Terminus) IHC-plus™ LS-B1518
Rabbit Polyclonal (IgG) to Human CTCF
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-CTCF Antibody (C-Terminus) IHC-plus™ LS-B10616
Rabbit Polyclonal (IgG) to Human CTCF
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CTCF
IHC - Paraffin, Immunoprecipitation


Human CTCF
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Immunoprecipitation

Specificity and Use

CTCF antibody was raised against a synthetic peptide mapping to a region between residues 150 and 200 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).
Species cross-reactivity: Human. Other species have not been tested. Reactivity with mouse (but not X. laevis) CTCF is expected owing to similarity of sequence between this ortholog and human CTCF at the site to which the epitope maps.
Immunohistochemistry: LS-B3184 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3184 was determined to be 5 mg/ml.


Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CTCF

P49711 NM_006565 NP_006556.1

CTCF Antibody, 11-zinc finger protein Antibody, CCCTC-binding factor Antibody, Transcriptional repressor CTCF Antibody, CTCFL paralog Antibody

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression.


Anti-CTCF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.


Anti-CTCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.


Anti-CTCF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3184 concentration 5 ug/ml.

Requested From: United States
Date Requested: 10/23/2016

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