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Anti-CSTF3 Antibody (clone 1D4) LS-C133142

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CSTF3 Antibodies

Anti-CSTF3 Antibody (aa556-585, AP) LS-C208686
Rabbit Polyclonal (IgG) to Human CSTF3
Human
Western blot, ELISA
AP Conjugated
Anti-CSTF3 Antibody (aa667-717) LS-C287925
Rabbit Polyclonal (IgG) to Human CSTF3
Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-CSTF3 Antibody (aa71-120) IHC-plus™ LS-B12595
Rabbit Polyclonal (IgG) to Human CSTF3
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1D4] (IgG1,k) to Human CSTF3
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CSTF3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1D4]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

CSTF3 antibody was raised against cSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CSTF3
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CSTF3

Q12996 NM_001326 NP_001317.1

CSTF3 Antibody, CSTF 77 kDa subunit Antibody, CF-1 77 kDa subunit Antibody, CSTF-77 Antibody

CSTF3 is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.
 

Requested From: United States
Date Requested: 12/3/2016

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