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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSF3 / G‑CSF Antibody LS‑C104779
Note: This antibody replaces LS-C54045, LS-C42075, LS-C15559
G-CSF antibody LS-C104779 is an unconjugated rabbit polyclonal antibody to mouse G-CSF (CSF3). Validated for ELISA, Neut and WB.
Catalog
Size
Price
LS-C104779-50
50 µg
Unavailable
LS-C104779-100
100 µg
Unavailable

Popular CSF3 / G-CSF Products

Antibody
Species: Human
Applications: Western blot, ELISA
Sandwich ELISA of CSF3 / G-CSF antibody LS-C104669
Species: Human
Applications: Western blot, ELISA, Neutralization
Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot, ELISA, Neutralization
Formalin-fixed, paraffin-embedded human tonsil stained with G-CSF antibody (CSF3/900). Note specific cytoplasmic staining of granulocytes.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

Product Description

G-CSF antibody LS-C104779 is an unconjugated rabbit polyclonal antibody to mouse G-CSF (CSF3). Validated for ELISA, Neut and WB.
About CSF3 / G-CSF
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. P09919 NM_000759 NP_000750.1

CSF3 Antibody, C17orf33 Antibody, Filgrastim Antibody, G-CSF Antibody, GCSF Antibody, Pluripoietin Antibody, CSF3OS Antibody, Lenograstim Antibody


Specifications

Target
Mouse CSF3 / G-CSF
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (5 - 10 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CSF3 / G-CSF antibody was raised against highly pure (>98%) recombinant mG-CSF.
Specificity
Mouse G-CSF
Usage
Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mG-CSF (0.5ng/ml), a concentration of 005-010 ug/ml of this antibody is required. ELISA: To detect mG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine G-CSF (LS-C104766) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF. Western Blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CSF3 / G-CSF antibody LS-C104779. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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