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CSF2 / GM‑CSF Antibody LS‑C15590
GM-CSF antibody LS-C15590 is an unconjugated rat monoclonal antibody to mouse GM-CSF (CSF2). Validated for ELISA and WB.
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Review This ProductPopular CSF2 / GM-CSF Products
Species:
Human
Applications:
IHC, IHC - Frozen, Western blot, ELISA, Functional Assay
Species:
Mouse
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, Functional Assay, Neutralization
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product Description
GM-CSF antibody LS-C15590 is an unconjugated rat monoclonal antibody to mouse GM-CSF (CSF2). Validated for ELISA and WB.
About CSF2 / GM-CSF
CSF2 / GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
P04141
NM_000758
NP_000749.2
CSF2 Antibody, Colony-stimulating factor Antibody, CSF Antibody, GM-CSF Antibody, Sargramostim Antibody, Molgramostin Antibody, GMCSF Antibody
Specifications
Target
Mouse CSF2 / GM-CSF
Host
Rat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
- Western blot
- ELISA
Immunogen
CSF2 / GM-CSF antibody was raised against yeast-expressed recombinant mouse GM-CSF.
Specificity
Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).
Usage
ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels.1,3-5 Biotinylated antibody should be paired with purified antibody (Cat. Western Blot: The purified antibody is also useful for immunoprecipitation4 and Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse GM-CSF under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Requested From:
Date Requested: 6/17/2018
Date Requested: 6/17/2018
