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Anti-CSF2 / GM-CSF Antibody (Azide-free) LS-C15586

Catalog	Size	Price
LS-C15586-500	500 µg	Unavailable

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Western Blot (reducing) of GM-CSF antibody LS-C104671. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-CSF2 / GM-CSF Antibody LS-C123657

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CSF2 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Antibody:	Rat Monoclonal (IgG2a,k) to Mouse CSF2 / GM-CSF
Reactivity:	Mouse
Application:	Western blot, ELISA, Neutralization
Format:	Unconjugated, Azide-free

Details

Target:	Mouse CSF2 / GM-CSF
Host:	Rat
Reactivity:	Mouse (tested or 100% immunogen sequence identity)
Clonality:	IgG2a,k Monoclonal
Conjugations:	Unconjugated
Purification:	Purified
Modifications:	Azide-free

Applications

Approved:

Western blot
ELISA
Neutralization

Specificity and Use

Immunogen:	CSF2 / GM-CSF antibody was raised against yeast-expressed recombinant mouse GM-CSF.
Specificity:	Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).
Usage:	ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse GM-CSF under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse GM-CSF bioactivity. A suitable LE/AF Rat IgG2a should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

Presentation:	PBS. No preservative added.
Storage:	Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions:	For research use only.

About CSF2 / GM-CSF

Uniprot: P04141 NCBI: NM_000758 NP_000749.2

Synonyms: CSF2 Antibody, Colony-stimulating factor Antibody, CSF Antibody, GM-CSF Antibody, Sargramostim Antibody, Molgramostin Antibody, GMCSF Antibody

Description: CSF2 / GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

(More About CSF2 / GM-CSF)

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Date Requested: 4/26/2018

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