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Anti-CSA / ERCC8 Antibody IHC-plus™ LS-B4076

Note: This antibody replaces LS-C109291

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CSA / ERCC8 Antibodies

Anti-CSA / ERCC8 Antibody IHC-plus™ LS-B4076
Rabbit Polyclonal to Human CSA / ERCC8
Human, Mouse, Rat, Bovine
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CSA / ERCC8 Antibody (aa210-238, AP) LS-C208891
Rabbit Polyclonal (IgG) to Human CSA / ERCC8
Human, Mouse
IHC, Western blot, ELISA
AP Conjugated
Anti-CSA / ERCC8 Antibody (aa210-238, FITC) LS-C208894
Rabbit Polyclonal (IgG) to Human CSA / ERCC8
Human, Mouse
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CSA / ERCC8
Human, Mouse, Rat, Bovine
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human CSA / ERCC8
Rabbit
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:3000)

Specificity and Use

CSA / ERCC8 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 106 and 300 of CSA.
Human ERCC8.
Cross reactivity with mouse has been confirmed in WB only. The suitability of the antibody for use in IHC with mouse samples has not been evaluated at this time. Cross-reactivity with rat and cow has not been experimentally confirmed. The antibody is predicted to cross-react with rat and cow based on sequence homologies of 96% and 95% respectively.

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About CSA / ERCC8

Q13216 NM_000082 NP_000073.1

ERCC8 Antibody, CKN1 Antibody, UVSS2 Antibody, CSA Antibody

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.

Immunohistochemistry

Immunohistochemistry
Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.

Western blot

Western blot
Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/2/2016

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