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Human CSA / ERCC8 | |
Rabbit | |
Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity) | |
Monkey, Bat (at least 90% immunogen sequence identity) | |
IgG Polyclonal | |
Unconjugated. Also available conjugated with Biotin, FITC, HRP. | |
Immunoaffinity purified | |
Unmodified |
CSA / ERCC8 antibody was raised against synthetic peptide located between aa72-121 of human ERCC8 (B2RB64, NP_000073). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Galago (92%); Marmoset, Bat (90%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (85%). | ||
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aa72-121 | ||
Human ERCC8 | ||
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. |
Lyophilized from PBS with 2% sucrose | |
Distilled water | |
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles. | |
For research use only. |
ERCC8 Antibody, CKN1 Antibody, UVSS2 Antibody, CSA Antibody
Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.
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