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CSA / ERCC8 Antibody (aa72‑121) LS‑C102730
ERCC8 antibody LS-C102730 is an unconjugated rabbit polyclonal antibody to ERCC8 (CSA) from human, mouse, rat and other species. Validated for WB.
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Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, Western blot, ELISA
Product Description
ERCC8 antibody LS-C102730 is an unconjugated rabbit polyclonal antibody to ERCC8 (CSA) from human, mouse, rat and other species. Validated for WB.
About CSA / ERCC8
Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.
Q13216
NM_000082
NP_000073.1
ERCC8 Antibody, CKN1 Antibody, UVSS2 Antibody, CSA Antibody
Specifications
Target
Human CSA / ERCC8
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
CSA / ERCC8 antibody was raised against synthetic peptide located between aa72-121 of human ERCC8 (B2RB64, NP_000073). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Galago (92%); Marmoset, Bat (90%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (85%).
Blocking Peptide
LS-E21196 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102730. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C102730. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa72-121
Specificity
Human ERCC8
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
ERCC8 antibody LS-C102730 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
ERCC8 antibody LS-C102730 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
ERCC8 antibody LS-C102730 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
ERCC8 antibody LS-C102730 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/25/2018
Date Requested: 6/25/2018
