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Catalog Number Size Price
LS-B10449-50 50 µl (1 mg/ml) $460 
CRYAB / Alpha B Crystallin Antibody - Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CRYAB / Alpha B Crystallin Antibody - IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
CRYAB / Alpha B Crystallin Antibody - Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody.
CRYAB / Alpha B Crystallin Antibody - Anti-CRYAB mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
CRYAB / Alpha B Crystallin Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal antibody.
CRYAB / Alpha B Crystallin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
CRYAB / Alpha B Crystallin Antibody - Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CRYAB / Alpha B Crystallin Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
CRYAB / Alpha B Crystallin Antibody - Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CRYAB / Alpha B Crystallin Antibody - IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
CRYAB / Alpha B Crystallin Antibody - Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody.
CRYAB / Alpha B Crystallin Antibody - Anti-CRYAB mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
CRYAB / Alpha B Crystallin Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal antibody.
CRYAB / Alpha B Crystallin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
CRYAB / Alpha B Crystallin Antibody - Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CRYAB / Alpha B Crystallin Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
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IHC‑plus™ Monoclonal Mouse anti‑Human CRYAB / Alpha B Crystallin Antibody (clone 6D11, IHC, IF, WB) LS‑B10449

IHC‑plus™ Monoclonal Mouse anti‑Human CRYAB / Alpha B Crystallin Antibody (clone 6D11, IHC, IF, WB) LS‑B10449

Note: This antibody replaces LS-C114680
Antibody:
CRYAB / Alpha B Crystallin Mouse anti-Human Monoclonal (6D11) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10449-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CRYAB / Alpha B Crystallin Mouse anti-Human Monoclonal (6D11) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha B Crystallin antibody LS-B10449 is an unconjugated mouse monoclonal antibody to Alpha B Crystallin (CRYAB) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CRYAB / Alpha B Crystallin
Synonyms
CRYAB | Alpha-crystallin B chain | CRYA2 | Crystallin, alpha B | CTPP2 | Heat shock protein beta-5 | HSPB5 | Alpha B Crystallin | Alpha(B)-crystallin | Rosenthal fiber component | Heat-shock 20 kD like-protein
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
6D11
Conjugations
Unconjugated
Purification
Protein A/G purified from tissue culture supernatant.
Modifications
Unmodified
Immunogen
Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
Specificity
Human CRYAB / Alpha B Crystallin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRYAB / Alpha B Crystallin
P02511 NM_001885 NP_001876.1

Validation

CRYAB / Alpha B Crystallin Antibody - Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CRYAB / Alpha B Crystallin Antibody (clone 6D11) for IHC, IF/Immunofluorescence, WB/Western, IP, Flow LS-B10449 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024