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Anti-CRYAB / Alpha B Crystallin Antibody (clone 6D11) IHC-plus™ LS-B10449

Note: This antibody replaces LS-C114680

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.75 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CRYAB / Alpha B Crystallin Antibody LS-B10449 Images

[click image for a larger image and more details]
Immunohistochemistry
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody.
Immunohistochemistry
Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart.
Immunofluorescence
Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody.
Immunofluorescence
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB...
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal...
Immunoprecipitation
Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP...
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid...

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6D11] (IgG1) to Human CRYAB / Alpha B Crystallin
Human, Monkey, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CRYAB / Alpha B Crystallin
Mouse
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6D11]
Protein A/G purified

Applications

IHC - Paraffin (10 µg/ml), Immunofluorescence (1:50 - 1:100), Western blot (1:500 - 1:1000), Immunoprecipitation, Flow Cytometry (1:100)

Specificity and Use

CRYAB / Alpha B Crystallin antibody was raised against full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
Human CRYAB / Alpha B Crystallin
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CRYAB / Alpha B Crystallin

P02511 NM_001885 NP_001876.1

CRYAB Antibody, Alpha-crystallin B chain Antibody, CRYA2 Antibody, Crystallin, alpha B Antibody, CTPP2 Antibody, Heat shock protein beta-5 Antibody, HSPB5 Antibody, Alpha B Crystallin Antibody, Alpha(B)-crystallin Antibody, Rosenthal fiber component Antibody, Heat-shock 20 kD like-protein Antibody


Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10449 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 7/6/2015

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