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Anti-CRYAB / Alpha B Crystallin Antibody (clone 6D11) IHC-plus™ LS-B10449

Note: This antibody replaces LS-C114680


Wt. Vol. Conc. Price
- 50 µl 0.75 mg/ml $395

LSBio (Direct) LSBio (Direct)

Other CRYAB / Alpha B Crystallin Products

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Mouse Monoclonal [clone 6D11] (IgG1) to Human CRYAB / Alpha B Crystallin
Human, Monkey, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry


Human CRYAB / Alpha B Crystallin
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6D11]
Protein A/G purified


IHC - Paraffin (10 µg/ml), Immunofluorescence (1:50 - 1:100), Western blot (1:500 - 1:1000), Immunoprecipitation, Flow Cytometry (1:100)

Specificity and Use

CRYAB / Alpha B Crystallin antibody was raised against full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
Human CRYAB / Alpha B Crystallin
Concentration: 0.5-1.0 mg/ml (Lot dependent)


PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CRYAB / Alpha B Crystallin

P02511 NM_001885 NP_001876.1

CRYAB Antibody, Alpha-crystallin B chain Antibody, CRYA2 Antibody, Crystallin, alpha B Antibody, CTPP2 Antibody, Heat shock protein beta-5 Antibody, HSPB5 Antibody, Alpha B Crystallin Antibody, Alpha(B)-crystallin Antibody, Rosenthal fiber component Antibody, Heat-shock 20 kD like-protein Antibody

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.


IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).


Anti-CRYAB / Alpha B Crystallin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10449 concentration 10 ug/ml.


Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody.


Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-CRYAB monoclonal antibody.


Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 11/29/2015

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