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Anti-CRYAA / Alpha A Crystallin Antibody (clone C9F2) LS-C93712
Rabbit Polyclonal to Human CRYAA / Alpha A Crystallin
Human
IHC, Western blot
Unconjugated
Mouse Monoclonal [clone C9F2] (IgG1) to Human CRYAA / Alpha A Crystallin
Human
Western blot, ELISA
Unconjugated
Details
Human CRYAA / Alpha A Crystallin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [C9F2]
Unconjugated
Protein G purified
Unmodified
Applications
Western blot (1:500 - 1:2000)
ELISA
Specificity and Use
Recombinant human kappa-Crystallin A protein Isotype Mouse IgG1, heavy chains and kappa light chains.
The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity. Since application vary, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500-1:2000. Recommended starting dilution: 1:1000.
Packaging
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
CRYAA Antibody, Alpha-crystallin A chain Antibody, Crystallin, alpha A Antibody, CRYA1 Antibody, Crystallin, alpha-1 Antibody, Heat shock protein beta-4 Antibody, HSPB4 Antibody
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.