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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRYAA / Alpha A Crystallin Antibody (clone C9F2) LS‑C93712
Alpha A Crystallin antibody LS-C93712 is an unconjugated mouse monoclonal antibody to human Alpha A Crystallin (CRYAA). Validated for ELISA and WB.
Catalog
Size
Price
LS-C93712-100
100 µg (1 mg/ml)
Unavailable

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Product Description

Alpha A Crystallin antibody LS-C93712 is an unconjugated mouse monoclonal antibody to human Alpha A Crystallin (CRYAA). Validated for ELISA and WB.
About CRYAA / Alpha A Crystallin
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. P02489 NM_000394 NP_000385.1

CRYAA Antibody, Alpha-crystallin A chain Antibody, Crystallin, alpha A Antibody, CRYA1 Antibody, Crystallin, alpha-1 Antibody, Heat shock protein beta-4 Antibody, HSPB4 Antibody


Specifications

Target
Human CRYAA / Alpha A Crystallin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [C9F2]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA
Immunogen
Recombinant human kappa-Crystallin A protein Isotype Mouse IgG1, heavy chains and kappa light chains.
Usage
The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity. Since application vary, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500-1:2000. Recommended starting dilution: 1:1000.
Presentation
PBS, pH 7.4, 0.1% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.

Western blot

Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.

Western blot

Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.

Western blot

Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.


Requested From: 
Date Requested: 6/19/2018

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