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Anti-CRYAA / Alpha A Crystallin Antibody (clone C9F2) LS-C93712

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CRYAA / Alpha A Crystallin Antibodies

Anti-CRYAA / Alpha A Crystallin Antibody (aa77-106, AP) LS-C255456
Rabbit Polyclonal (IgG) to Human CRYAA / Alpha A Crystallin
Human
Western blot, ELISA
AP Conjugated
Anti-CRYAA / Alpha A Crystallin Antibody (aa77-106, Biotin) LS-C255458
Rabbit Polyclonal (IgG) to Human CRYAA / Alpha A Crystallin
Human
Western blot, ELISA
Biotin Conjugated
Anti-CRYAA / Alpha A Crystallin Antibody (aa77-106, PE) LS-C255461
Rabbit Polyclonal (IgG) to Human CRYAA / Alpha A Crystallin
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone C9F2] (IgG1) to Human CRYAA / Alpha A Crystallin
Human
Western blot, ELISA
Unconjugated

Details

Human CRYAA / Alpha A Crystallin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [C9F2]
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

CRYAA / Alpha A Crystallin antibody was raised against recombinant human kappa-Crystallin A protein Isotype Mouse IgG1, heavy chains and kappa light chains.
The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity. Since application vary, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500-1:2000. Recommended starting dilution: 1:1000.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CRYAA / Alpha A Crystallin

P02489 NM_000394 NP_000385.1

CRYAA Antibody, Alpha-crystallin A chain Antibody, Crystallin, alpha A Antibody, CRYA1 Antibody, Crystallin, alpha-1 Antibody, Heat shock protein beta-4 Antibody, HSPB4 Antibody

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.

Western blot

Western blot
Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin a?(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline a?(~20 kD). This antibody is not shown cross-activity.
 

Requested From: United States
Date Requested: 12/8/2016

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