Human CRISP2 / TSP1
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
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CRISP2 / TSP1 antibody was raised against synthetic peptide C-QHSDPEDRKTSTR from an internal region of human CRISP2 (NP_003287.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (92%); Rabbit (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8317. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human CRISP2. Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 ug/ml. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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