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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRISP2 / TSP1 Antibody (aa81‑93) IHC‑plus™ LS‑B8317
Note: This antibody replaces LS-C112930
TSP1 antibody LS-B8317 is an unconjugated goat polyclonal antibody to human TSP1 (CRISP2). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8317-50
50 µg (0.5 mg/ml)
Unavailable

Popular CRISP2 / TSP1 Products

Anti-CRISP2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5318 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

TSP1 antibody LS-B8317 is an unconjugated goat polyclonal antibody to human TSP1 (CRISP2). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About CRISP2 / TSP1
May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation. P16562 NM_003296 NP_003287.1

CRISP2 Antibody, CRISP-2 Antibody, GAPDL5 Antibody, Testis-specific protein TPX-1 Antibody, TPX1 Antibody, TSP1 Antibody, Cancer/testis antigen 36 Antibody, CT36 Antibody


Specifications

Target
Human CRISP2 / TSP1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CRISP2 / TSP1 antibody was raised against synthetic peptide C-QHSDPEDRKTSTR from an internal region of human CRISP2 (NP_003287.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (92%); Rabbit (85%).
Blocking Peptide
LS-E27290 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8317. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa81-93
Specificity
Human CRISP2. Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 ug/ml. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.
Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane

Immunohistochemistry

Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.
Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane

Immunohistochemistry

Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.
Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane

Immunohistochemistry

Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.
Anti-CRISP-2 / TSP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8317 dilution 3.75 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane


Requested From: 
Date Requested: 6/25/2018

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