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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRHR2 / CRF2 Receptor Antibody (aa243‑272) LS‑C167039
CRF2 Receptor antibody LS-C167039 is an unconjugated rabbit polyclonal antibody to CRF2 Receptor (CRHR2) from human and mouse. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C167039-400
400 µl
Unavailable

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Product Description

CRF2 Receptor antibody LS-C167039 is an unconjugated rabbit polyclonal antibody to CRF2 Receptor (CRHR2) from human and mouse. Validated for Flow, IF, IHC and WB.
About CRHR2 / CRF2 Receptor
CRHR2 / CRF2 Receptor belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Q13324 NM_001883 NP_001874.2

CRHR2 Antibody, CRF-R-2 Antibody, CRF-R2 Antibody, CRF2R Antibody, Crh2 receptor Antibody, CRF2 receptor, beta isoform Antibody, CRFR2 Antibody, CRFR-2 Antibody, CRH-R-2 Antibody, CRH2R Antibody, CRF-RB Antibody, CRF2 Antibody, CRH-R2 Antibody, HM-CRF Antibody


Specifications

Target
Human CRHR2 / CRF2 Receptor
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8459 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167039. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa243-272
Specificity
This CRHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human CRHR2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.
Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.

Flow Cytometry

CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.
Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.

Flow Cytometry

CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.
Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.

Flow Cytometry

CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CRHR2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CRHR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.
Western blot of CRHR2 Antibody in HeLa cell line lysates (35 ug/lane). CRHR2 (arrow) was detected using the purified antibody.

Flow Cytometry

CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRHR2 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/25/2018

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