Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Bovine, Horse, Pig, Zebrafish (at least 90% immunogen sequence identity)
Western blot (0.1 - 0.3 µg/ml)
IHC - Paraffin
Specificity and Use
CRFR1 / CRHR1 antibody was raised against synthetic peptide C-NEEKKSKVHYH from an internal region of human CRHR1 / CRF1 (NP_004373.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant (100%); Bovine, Horse, Pig, Zebrafish (91%); Panda, Dog, Bat, Opossum, Turkey, Lizard, Frog (82%).
G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H.