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Anti-CREB1 / CREB Antibody (phospho-Ser133) LS-C60073

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CREB1 / CREB Antibodies

Anti-CREB1 / CREB Antibody (phospho-Ser133) LS-C248
Rabbit Polyclonal to Rat CREB1 / CREB
Rat, Human, Mouse, Bovine, Dog, Chicken, Xenopus
Western blot
Unconjugated
Western blot Image
Anti-CREB1 / CREB Antibody (aa251-300) LS-C110441
Rabbit Polyclonal to Human CREB1 / CREB
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-CREB1 / CREB Antibody (clone 5G3) LS-C169350
Mouse Monoclonal [clone 5G3] (IgG1) to Human CREB1 / CREB
Human, Monkey, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CREB1 / CREB
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CREB1 / CREB
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:6000)

Specificity and Use

CREB1 / CREB antibody was raised against cREB phosphopeptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
pSer133
Antiserum was first sequentially pre-adsorbed against a control E. coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with nonphosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat
This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1 %) by ELISA against the non-phosphorylated form of the immunizing peptide. This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates. Bands of 46 and 43 kD corresponding to phosphorylated CREB are observed in western blots. Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB. No cross-reactivity occurs with non-phosphorylated CREB. For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining. Although not tested this antibody is likely functional by FACS and immunoprecipitation.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About CREB1 / CREB

P16220 NM_134442 NP_004370.1

CREB1 Antibody, CREB-1 Antibody, Transactivator protein Antibody, CREB Antibody

CREB (cAMP response element binding protein1), a DNA binding protein, is a member of the leucine zipper family of transcription factors. CREB interaction with DNA requires phosphorylation at Ser 119 by activated cAMP protein kinase A. Once phosphorylated CREB binds to an 8 base enhancer know as the cAMP response element (CRE) containing a conserved core sequence TGACG. CREB also interacts with CBP (CREB binding protein) and RNA polymerase II when phosphorylated on Ser 133.

Western blot

Western blot
Anti-CREB pS133 Antibody - Western Blot. Anti-CREB pS133 was used to detect phosphorylated CREB by western blot. Recombinant His-tagged human CREB was produced in E. coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of indicated amounts of control (-) and in-vitro phosphorylated CREB (P) were loaded to show that the antibody reacts specifically with the phosphorylated form. Blots were blocked in 5% milk in TBS+0.1% Tween-20 (TBST-M) overnight at 4C. Detection occurs using a 1:500 dilution of antibody diluted in TBST-M and incubated at room temperature with rocking for 1 hour. Blots were rinsed 6X with TBST and incubated with goat anti-rabbit-HRP at 1:5000 in TBST-M at room temperature for 45 min. Blots were again rinsed 6X with TBST and then processed using ECL reagent (Amersham) according to manufacturers instructions. Exposure time: 1 min using Kodak Biomax MR film. Personal Communication, R. Screaton, The Salk Institute for Biological Studies.

Western blot

Western blot
Anti-CREB pS133 Antibody - Western Blot. Anti-CREB pS133 was used to detect phosphorylated CREB by western blot. Recombinant His-tagged human CREB was produced in E. coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Pan reactive CREB (# LS-C19089) reacts equally with both non-phosphorylated and phosphorylated CREB (not shown). Detection occurs using a 1:500 dilution of antibody followed by 1:5000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.

Requested From: United States
Date Requested: 12/8/2016

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