Human CREB1 / CREB

Rabbit

Human, Monkey, Bat, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE, AF488.

Protein A purified

Unmodified

KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%); Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%); Rat, Xenopus (90%).

aa5-24

Recognizes human CREB at 43kD. Species cross-reactivity: Broad species cross-reactivity expected due to conservation of immunogenic sequence.

• Western blot
• Immunoprecipitation

Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 mg/ml detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5 ul of 2- mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. Immunoprecipitation: 5 mg (will immunoprecipitated CREB from 500 mg of HeLa nuclear extract).

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.1% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16220 NCBI: NM_134442 NP_004370.1