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Anti-CRADD / RAIDD Antibody IHC-plus™ LS-B3738

Note: This antibody replaces LS-C63136

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CRADD / RAIDD Antibodies

Anti-CRADD / RAIDD Antibody (C-Terminus) LS-C256
Rabbit Polyclonal to Human CRADD / RAIDD
Human
IHC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CRADD / RAIDD Antibody (clone 4B12) LS-C179788
Mouse Monoclonal [clone 4B12] (IgG1) to Human CRADD / RAIDD
Human
Western blot
Unconjugated
Western blot Image
Anti-CRADD / RAIDD Antibody (aa1-199) IHC-plus™ LS-B13801
Rabbit Polyclonal (IgG) to Human CRADD / RAIDD
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CRADD / RAIDD
Human, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human CRADD / RAIDD
Rabbit
Human, Dog (tested or 100% immunogen sequence identity)
Monkey, Hamster (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500)

Specificity and Use

CRADD / RAIDD antibody was raised against a 15 residue synthetic peptide (C)NSSPSDRQINQLAQR based on the human RAIDD (residues 112-126) (1) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Dog (100%); Marmoset, Hamster, Elephant, Panda (93%); Monkey, Mouse (87%).
Detects an ~24 kD protein, corresponding to the apparent molecular mass of RAIDD on SDS-PAGE immunoblots. This 24 kD band, as well as a 67 kD band, can be specifically inhibited by the relevant peptide.
Immunohistochemistry: LS-B3738 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3738 was determined to be 10 ug/ml.

Packaging

BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
Store at -20°C.
For research use only.

About CRADD / RAIDD

P78560 NM_003805 NP_003796.1

CRADD Antibody, Death adaptor molecule RAIDD Antibody, RAIDD Antibody, MRT34 Antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules.

Immunohistochemistry

Immunohistochemistry
Anti-CRADD / RAIDD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3738 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CRADD / RAIDD antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3738 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CRADD / RAIDD antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3738 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/5/2016

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