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Anti-CRABP1 / CRABP Antibody (clone AT1A1) IHC-plus™ LS-B3590

Note: This antibody replaces LS-C103498

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml Unavailable

Most Popular CRABP1 / CRABP Antibodies

Anti-CRABP1 / CRABP Antibody (clone AT1A1) IHC-plus™ LS-B3590
Mouse Monoclonal [clone AT1A1] (IgG2b,k) to Human CRABP1 / CRABP
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CRABP1 / CRABP Antibody (aa90-118) LS-C167019
Rabbit Polyclonal to Human CRABP1 / CRABP
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CRABP1 / CRABP Antibody IHC-plus™ LS-B13800
Rabbit Polyclonal (IgG) to Human CRABP1 / CRABP
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AT1A1] (IgG2b,k) to Human CRABP1 / CRABP
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CRABP1 / CRABP
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT1A1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

CRABP1 / CRABP antibody was raised against recombinant human CRABP1 (1-137aa) purified from E. coli
human CRABP1 (1-137aa)
Immunohistochemistry: LS-B3590 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3590 was determined to be 5 ug/ml. Western Blot: Extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About CRABP1 / CRABP

P29762 NM_004378 NP_004369.1

CRABP1 Antibody, CRABP-I Antibody, CRABPI Antibody, CRABP Antibody

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid-binding protein is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. CRABP1 is constitutively expressed and is believed to have different functions in the cell than the related CRABP2.

Immunohistochemistry

Immunohistochemistry
Anti-CRABP1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3590 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CRABP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3590 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CRABP1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3590 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of mouse eye extract.

Requested From: 
Date Requested: 3/29/2017

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