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Anti-CR1 / CD35 Antibody (clone E11, Azide-free) IHC-plus™ LS-B2556

Note: This antibody replaces LS-C45218

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CR1 / CD35 Antibodies

Anti-CR1 / CD35 Antibody (FITC) LS-C4989
Mouse Monoclonal (IgG1) to Human CR1 / CD35
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
FITC Conjugated
Anti-CR1 / CD35 Antibody (clone 5H3/B1) LS-C88946
Mouse Monoclonal [clone 5H3/B1] (IgG1) to Human CR1 / CD35
Human
ELISA
Unconjugated
Anti-CR1 / CD35 Antibody LS-C121517
Mouse Monoclonal (IgG1) to Human CR1 / CD35
Human, Baboon, Monkey, Cynomolgus
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone E11] (IgG1) to Human CR1 / CD35
Human, Baboon, Cynomolgus monkey, Rhesus monkey
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated, Azide-free

Details

Human CR1 / CD35
Mouse
Human, Baboon, Cynomolgus monkey, Rhesus monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [E11]
Unconjugated
Protein A purified
Azide-free. Also available Unmodified.

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Western blot
  • Flow Cytometry (1:50 - 1:100)
  • (applications tested for the base form of this product only)

Specificity and Use

CR1 / CD35 antibody was raised against human acute monocytic leukaemia cells.
Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.

Packaging

PBS, pH 7.4. Sourced in TCS.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CR1 / CD35

P17927 NM_000651 NP_000642.3

CR1 Antibody, C3b/C4b receptor Antibody, C3-binding protein Antibody, CD35 Antibody, C3BR Antibody, Knops blood group antigen Antibody, C4BR Antibody, CD35 antigen Antibody, Complement receptor type 1 Antibody, KN Antibody

CR1 / CD35 is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement.

Immunohistochemistry

Immunohistochemistry
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7301 concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2556 concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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