Human CR1 / CD35

Mouse

Human, Monkey (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

CR1/802

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Recombinant human protein.

Human CR1 / CD35

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This mAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma. Optimal dilution of the CD35 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17927 NCBI: NM_000651 NP_000642.3