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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CPXCR1 / CT77 Antibody (aa396‑445) IHC‑plus™ LS‑B4030 Best Seller
Note: This antibody replaces LS-C101792
CT77 antibody LS-B4030 is an unconjugated rabbit polyclonal antibody to human CT77 (CPXCR1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4030-50
50 µg
Unavailable

Popular CPXCR1 / CT77 Products

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at dilution of 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee
Applications: IHC, IHC - Paraffin, Western blot
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee
Applications: IHC, IHC - Paraffin, Western blot
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CT77 antibody LS-B4030 is an unconjugated rabbit polyclonal antibody to human CT77 (CPXCR1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CPXCR1 / CT77
CPXCR1 / CT77 is one of several genes identified in a region of the X chromosome associated with an X-linked cleft palate (CPX) disorder. The encoded protein contains a motif similar to a motif found in zinc-finger proteins. Mutation analysis of this gene has not revealed any mutation which causes the CPX disorder. Multiple alternatively spliced variants, encoding the same protein, have been identified. Q8N123 NM_033048 NP_149037.4

CPXCR1 Antibody, Cancer/testis antigen 77 Antibody, CT77 Antibody


Specifications

Target
Human CPXCR1 / CT77
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CPXCR1 / CT77 antibody was raised against synthetic peptide located between aa396-445 of human CPXCR1 (Q8N123, NP_149037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey (85%).
Blocking Peptide
LS-E14415 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4030. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa396-445
Specificity
Human CPXCR1
Usage
Immunohistochemistry: LS-B4030 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4030 was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPXCR1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4030 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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