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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CPN1 Antibody (aa251‑300) IHC‑plus™ LS‑B5828 Best Seller
Note: This antibody replaces LS-C102408
CPN1 antibody LS-B5828 is an unconjugated rabbit polyclonal antibody to CPN1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5828-50
50 µg (1 mg/ml)
Unavailable

Popular CPN1 Products

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CPN1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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Product Description

CPN1 antibody LS-B5828 is an unconjugated rabbit polyclonal antibody to CPN1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CPN1
Carboxypeptidase N (CPN)is a plasma metallo-protease that cleaves basic amino acid residues from the C terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and is also known as kininase-1 and anaphylatoxin inactivator. P15169 NM_001308 NP_001299.1

CPN1 Antibody, ACBP Antibody, Arginine carboxypeptidase Antibody, Anaphylatoxin inactivator Antibody, Carboxypeptidase K Antibody, Kininase-1 Antibody, Lysine carboxypeptidase Antibody, Kininase I Antibody, Serum carboxypeptidase N Antibody, SCPN Antibody, Plasma carboxypeptidase B Antibody, CPN Antibody


Specifications

Target
Human CPN1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CPN1 antibody was raised against synthetic peptide located between aa251-300 of human CPN1 (P15169, NP_001299). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Marmoset, Opossum, Turkey, Zebra finch, Chicken (92%); Lizard, Zebrafish (84%); Drosophila (81%).
Blocking Peptide
LS-E18637 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5828. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa251-300
Specificity
Human CPN1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CPN1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5828 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CPN1 antibody LS-B5828 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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