Human CPA2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived recombinant human rhCPA2.

Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%.

• Western blot (1 - 2 µg/ml)
• Immunoprecipitation (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)
• Neutralization

Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Western blot:1-2 ug/ml the detection limit is ~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at ~1.5 ug/ml (IC50) under conditions in which the enzyme was. present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the. molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at. approximately 5:1 molar ratio of the antibody to the enzyme. Immunoprecipitation: 25 ug/ml. Direct ELISA: 0.5-1.0 ug/mL.

Lyophilized PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48052 NCBI: NM_001869 NP_001860.2