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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CPA1 / Carboxypeptidase A Antibody (clone 3F11) LS‑C196955
Carboxypeptidase A antibody LS-C196955 is an unconjugated mouse monoclonal antibody to human Carboxypeptidase A (CPA1). Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C196955-100
100 µg
Unavailable

Popular CPA1 / Carboxypeptidase A Products

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Product Description

Carboxypeptidase A antibody LS-C196955 is an unconjugated mouse monoclonal antibody to human Carboxypeptidase A (CPA1). Validated for ELISA, IP and WB.
About CPA1 / Carboxypeptidase A
CPA1 / Carboxypeptidase A is a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. P15085 NM_001868 NP_001859.1

CPA1 Antibody, CPA Antibody, Carboxypeptidase A1 Antibody, Pancreatic carboxypeptidase A Antibody, Carboxypeptidase A Antibody


Specifications

Target
Human CPA1 / Carboxypeptidase A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3F11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
CPA1 (AAH05279, aa1-419 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CPA1 / Carboxypeptidase A
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.
Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.
Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.
Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody (M01), clone 3F11.Lane 1: CPA1 transfected lysate(47.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.
Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPA1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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