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Anti-Complement C3 Antibody IHC-plus™ LS-B2545

Note: This antibody replaces LS-C22287


Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

Complement C3 Antibody LS-B2545 Images

[click image for a larger image and more details]
Anti-Complement C3 antibody IHC of human liver.

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Mouse Monoclonal (IgG1,k) to Human Complement C3
IHC - Paraffin, Western blot, ELISA


Human Complement C3
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Protein A/G purified


IHC - Paraffin (10 µg/ml), Western blot, ELISA (1:30000)

Specificity and Use

Complement C3 antibody was raised against c3 isolated from human plasma.
Recognizes Complement component C3 (human). Epitope specificity differs from that of clones 6F66 and 6F63 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against C3. In Western blotting after SDS-PAGE, this antibody reacts with C3 in reduced as well as non-reduced forms.
Immunohistochemistry: LS-B2545 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2545 was determined to be 10 ug/ml.


PBS, pH 7.4, 15 mM sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Complement C3

P01024 NM_000064 NP_000055.2

C3 Antibody, AHUS5 Antibody, ARMD9 Antibody, Complement component C3 Antibody, CPAMD1 Antibody, Complement C3 Antibody, ASP Antibody, Complement component 3 Antibody

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process.


Anti-Complement C3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2545 concentration 10 ug/ml.

Requested From: United States
Date Requested: 10/6/2015

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