Human Complement C3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

10-21

Unconjugated

Protein A purified

Unmodified

Native purified human C3a.

Recognizes 9 kD complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer. Shows minimal reactivity with other complement proteins in radioimmunoassays and hemagglutination. Clone 10-21 has been reported to work in western blotting applications.

• Western blot
• ELISA (1:1500 - 1:6000)

BBS, 0.1% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01024 NCBI: NM_000064 NP_000055.2