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Anti-Complement C3 Antibody (clone H13) LS-C22305

Note: This antibody replaces LS-C22292, LS-C123298


Wt. Vol. Conc. Price
50 µg - - Unavailable

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Mouse Monoclonal [clone H13] (IgG2b) to Human Complement C3
Human, Guinea pig, Rabbit
IHC - Frozen, Western blot


Human Complement C3
Human, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [H13]
Protein A purified


  • IHC - Frozen
  • Western blot

Specificity and Use

Complement C3 antibody was raised against human complement component C3.
Recognizes a C3a-determinant (C3 alpha-chain) which is present also on native C3. Allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with this clone. The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited; similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. Species cross-reactivity: Guinea pig and rabbit C3/C3a.
Suitable for use in Immunohistochemistry (frozen sections) and Western blotting. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays research purposes.


Lyophilized from PBS, pH 7.4, 0.5% BSA, 0.9% sodium azide
1 ml sterile ddH20
Lyophilized and resuspended forms: -20°C
For research use only.

About Complement C3

P01024 NM_000064 NP_000055.2

C3 Antibody, AHUS5 Antibody, ARMD9 Antibody, Complement component C3 Antibody, CPAMD1 Antibody, Complement C3 Antibody, ASP Antibody, Complement component 3 Antibody

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process.

Requested From: 
Date Requested: 4/25/2017

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