Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 2D8] (IgG1,k) to Human Complement C3
IHC - Paraffin, Western blot, ELISA
Mouse Monoclonal [clone 7C10] (IgG1,k) to Human Complement C3
Western blot, ELISA
Human Complement C3
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [7C10]
Protein A/G purified
Western blot (1:1000)
Specificity and Use
Complement C3 antibody was raised against c3 isolated from human plasma.
This antibody reacts strongly with C3. Strong reaction is seen in ELISA with human C3 coated directly onto the microtiter well, or when used as detection antibody in sandwich ELISA in combination with a polyclonal C3 antibody. It also reacts with C3b deposited on coated antibody molecules. In Western blotting, it reacts with C3 in both reduced and unreduced forms.
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process.