Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 11H9] (IgG2a) to Mouse Complement C3
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Mouse Complement C3
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [11H9]
IHC - Frozen
Western blot (1:25)
Flow Cytometry (1:25)
Specificity and Use
Complement C3 antibody was raised against mouse Complement C3.
Recognizes mouse C3, C3b, iC3b and C3d.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. ELISA: Coating antibody. Western Blot: 1:25. Flow Cytometry: 1:25. Immunohistochemistry (Frozen sections): 1:25.
PBS, 0.02% sodium azide, 0.1% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
Autoantibody-mediated complement C3a receptor activation contributes to the pathogenesis of preeclampsia. Wang W, Irani RA, Zhang Y, Ramin SM, Blackwell SC, Tao L, Kellems RE, Xia Y. Hypertension. 2012 60:712-21. (IHC; Mouse)[Full Text Article]
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process.